Journal: eLife

Article Title: Regulative synthesis of capsular polysaccharides in the pathogenesis of Streptococcus suis

doi: 10.7554/eLife.101760

Figure Lengend Snippet: Mice were inoculated with acetic acid through the nostril, and 1 hr later, infected intranasally (i.n.) with 05ZYH33. ( A ) Sagittal views of the olfactory system. Sections of the distal nasal cavity and olfactory epithelium from uninfected (a, c, and e) and 05ZYH33-infected (b, d, and f) mice. Red or orange, 05ZYH33; green, the neuronal marker β-tubulin III; blue, DNA. NC, nasal cavity; OE, olfactory epithelium; LP, lamina propria; CP, cribriform plate; OB, olfactory bulb. ( B ) A schematic drawing of the sagittal plane of the rodent nose elucidates the compartments of the olfactory bulb (OB), olfactory epithelium (OE), nasal cavity (NC), and brain. This panel is redrawn from ‘Mouse Olfactory System’ ( inspiredpencil.com ). The dotted line indicates the anteroposterior localization of the coronal sections in C. ( C ) One hour or nine days after 05ZYH33 infection, coronal brain sections were prepared and stained with hematoxylin and eosin (H&E). The sections display the regions of the ventral striatum and basal forebrain located behind the anterior olfactory nucleus. Arrows indicate infiltrated inflammatory cells in the lower areas of the ventral striatum or basal forebrain. ( D, E ) Mice were inoculated with acetic acid or phosphate-buffered saline (PBS), and 1 hr later infected i.n. with 05ZYH33 or ∆2BSS2. ( D ) Colony-forming units (CFUs) of 05ZYH33 or ( E ) ∆2BSS2 in nasal-associated lymphoid tissue (NALT), the cerebrospinal fluid (CSF), and blood were determined 1 hr after infection.

Article Snippet: Immunostaining was conducted using a primary rabbit anti- S. suis antibody (1:100) and a mouse anti-tubulin beta III antibody (Merck Millipore, Darmstadt, Germany catalog no. mab5564, 1:300) to detect neuronal structures, and then appropriate fluorophore-conjugated secondary antibodies: donkey anti-rabbit Cy3 (Jackson ImmunoResearch Laboratories, Ely, UK, catalog no. 711-166-152, 1:1000), donkey anti-mouse FITC (Jackson ImmunoResearch Laboratories, Ely, UK, catalog no. 715-096-150, 1:500).

Techniques: Infection, Marker, Staining, Saline

Journal: Cell death discovery

Article Title: Pkd2l1 deletion inhibits the neurogenesis of cerebrospinal fluid-contacting neurons and impedes spinal cord injury repair.

doi: 10.1038/s41420-025-02492-y

Figure Lengend Snippet: Fig. 1 Pkd2l1+ CSF-cNs have the characteristics of both immature neurons and adult NSCs. a Immunofluorescence staining showing expression of the NSC marker Nestin in Pkd2l1+ CSF-cNs. b Pkd2l1+ CSF-cNs expressed the NSC marker Sox2 by immunofluorescence staining. c Pkd2l1+ CSF-cNs expressed the NSC marker CD133 by immunofluorescence staining. d Pkd2l1+ CSF-cNs did not express the NSC marker GFAP by immunofluorescence staining. e Pkd2l1+ CSF-cNs expressed the neuroblast marker doublecortin (Dcx) by immunofluorescence staining. f Immunofluorescence staining showed that Pkd2l1+ CSF-cNs expressed the immature neuronal marker βIII-tubulin (red). Scale bars, 20 µm. Nuclear counterstaining, DAPI.

Article Snippet: Subsequently, the specimens were treated with a solution containing 0.25% Triton X-100 for 10min, followed by blocking with 1% BSA for 1 h. The sections were incubated with primary antibodies against the following proteins at 4 °C: Pkd2l1 (AB9084, 1:700, Millipore, USA), Nestin (sc23927, 1:200, Santa Cruz Biotechnology, Dallas, TX, USA), Sox2 (ab79351, 1:500, abcam, USA), Dcx (ab153668, 1:200, abcam, USA), β-III tubulin (66240-1-Ig, 1:200, Proteintech, USA), GFAP (16825-1-AP, 1:300, Proteintech, USA), Foxj1 (sc-53139, 1:200, Santa Cruz Biotechnology, Dallas, TX, USA), GABA (A2052, 1:200, Millipore, USA), Gata2 (11103-1-AP, 1:300, Proteintech, USA), and Gata3 (66400-1-Ig, 1:300, Proteintech, USA).

Techniques: Staining, Expressing, Marker

Journal: bioRxiv

Article Title: ER fusogens maintain membrane reservoir to ensure brain function

doi: 10.1101/2025.04.12.648519

Figure Lengend Snippet: A: Distributions of offspring from intercrosses between ATL2 gt/+ mice. B: H&E staining and β-III-tubulin immunohistochemical staining of control and ATL2 gt/gt embryos at E16.5 (scale bars, 2 mm). C: Distributions of offspring from intercrosses between ATL2 fl/fl and ATL2 fl/wt Nes-Cre mice. D: Footprint assay of control and ATL2 fl/fl Nes-Cre mice. E: Image of control and ATL2 fl/fl Nes-Cre mice (scale bar, 2 cm). F: Image and tissue weights of brains of control and ATL2 fl/fl Nes-Cre mice (scale bar, 0.5 cm). G: H&E staining of the sagittal sections of brains from control and ATL2 fl/fl Nes-Cre mice (scale bars, 1 mm). Data are represented as mean ± SD. The statistical significance of mean values was analyzed using an unpaired Student’s t test, **** p < 0.0001.

Article Snippet: Proteins were detected using the following specific antibodies: ATL1 (Sigma), ATL2 (Abcam, Proteintech), ATL3 (Proteintech) GFAP (Proteintech), NeuN (Sigma), beta-actin (Proteintech), BLBP (Abcam), beta-III-tubulin (Proteintech), calbindin (Abcam, Proteintech), FDFT1 (Abcam).

Techniques: Staining, Immunohistochemical staining, Control